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(A–D) Protein levels of the phosphorylated and total forms of <t>JAK1,</t> JAK2, and STAT1 in mouse liver tissue (A, B) and macrophages (C, D) detected by Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Symbols “+” and “–” denote the presence or absence of MSA/IFN-γ in the culture medium, respectively. ConA, concanavalin A; MSA, mesaconate; IFN-γ, interferon-gamma; JAK1/2, Janus tyrosine kinase 1/2; STAT1, signal transducer and activator of transcription 1; phos-JAK1/2, phosphorylated JAK1/2; phos-STAT1, phosphorylated STAT1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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(A–D) Protein levels of the phosphorylated and total forms of JAK1, JAK2, and STAT1 in mouse liver tissue (A, B) and macrophages (C, D) detected by Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Symbols “+” and “–” denote the presence or absence of MSA/IFN-γ in the culture medium, respectively. ConA, concanavalin A; MSA, mesaconate; IFN-γ, interferon-gamma; JAK1/2, Janus tyrosine kinase 1/2; STAT1, signal transducer and activator of transcription 1; phos-JAK1/2, phosphorylated JAK1/2; phos-STAT1, phosphorylated STAT1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Journal: Journal of Clinical and Translational Hepatology

Article Title: Protective Effect of Mesaconate on Autoimmune Hepatitis via Suppression of Inflammatory Response and Oxidative Stress

doi: 10.14218/JCTH.2025.00112

Figure Lengend Snippet: (A–D) Protein levels of the phosphorylated and total forms of JAK1, JAK2, and STAT1 in mouse liver tissue (A, B) and macrophages (C, D) detected by Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Symbols “+” and “–” denote the presence or absence of MSA/IFN-γ in the culture medium, respectively. ConA, concanavalin A; MSA, mesaconate; IFN-γ, interferon-gamma; JAK1/2, Janus tyrosine kinase 1/2; STAT1, signal transducer and activator of transcription 1; phos-JAK1/2, phosphorylated JAK1/2; phos-STAT1, phosphorylated STAT1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Antibodies used included: anti-GAPDH (AB0037, Abways), anti-IL-1β (26048-4-AP, Proteintech), anti-apoptotic B-cell lymphoma 2 (26593-1-AP, Proteintech), anti-Bcl2-associated X protein (50599-2-Ig, Proteintech), anti-cleaved-caspase-3 (#9661, Cell Signaling Technology (CST)), anti-cleaved-poly ADP ribose polymerase (#5625, CST), anti-inducible nitric oxide synthase (18985-1-AP, Proteintech), anti-Janus kinase 1 (JAK1) (66466-1-Ig, Proteintech), anti-phosphorylated JAK1 (phos-JAK1) (#74129, CST), anti-JAK2 (#3230, CST), anti-phosphorylated JAK2 (phos-JAK2) (#3771, CST), anti-signal transducer and activator of transcription 1 (STAT1) (10144-2-AP, Proteintech), and anti-phosphorylated STAT1 (phos-STAT1) (28977-1-AP, Proteintech).

Techniques: Western Blot